Design of Tunable Oscillatory Dynamics in a Synthetic NF-kB Signaling Circuit.
Cell Syst.. 2017 Nov 22
Abstract. PDF.
Tracing Information Flow from Erk to Target Gene Induction Reveals Mechanisms of Dynamic and Combinatorial Control.
Mol Cell. 2017 Sep 7
Abstract. PDF.
Engineering Therapeutic T cells: From Synthetic Biology to Clinical Trials.
Annu Rev Pathol. 2017 Jan
Abstract. PDF.
Interrogating cellular perception and decision making with optogenetic tools.
J Cell Biol. 2017 April 7
Abstract. PDF.
CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells.
Scientific Reports. 2017 April 7
Abstract. PDF.
The Principles of Engineering Immune Cells to Treat Cancer.
Cell. 2017 February 9
Abstract. PDF.
Complex transcriptional modulation with orthogonal and inducible dCas9 regulators.
Nat Methods. 2016 December
Abstract. PDF.
Engineering dynamical control of cell fate switching using synthetic phospho-regulons.
Proceedings of the National Academy of Sciences. 2016 November 22
Abstract. PDF.
Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of mutiple genomic loci.
Nucleic Acids Research. 2016 January 5
Abstract. PDF.
Engineering T cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors.
Cell. 2016 October 6
Abstract. PDF.
Cellular perception and misperception: Internal models for decision-making shaped by evolutionary experience.
Bioessays. 2016 July 27
Abstract. PDF.
Modular engineering of cellular signaling proteins and networks.
Curr Opin Struct Biol. 2016 July 13
Abstract. PDF.
CRISPR/Cas9 for Human Genome Engineering and Disease Research.
Annu Rev Genomics Hum Genet. 2016 May 23
Abstract. PDF.
Nucleosome breathing and remodeling constrain CRISPR-Cas9 function.
eLife. 2016; 5:e13450 Apr 28
Abstract. PDF.
Precision tumor recognition by T cells with combinatorial antigen-sensing circuits.
Cell. 2016 Feb. 11
Abstract. PDF. Supplemental Info.
Engineering customized cell sensing and response behaviors using synthetic notch receptors.
Cell. 2016 Feb. 11
Abstract. PDF. Supplemental Info. Addgene.
*These authors contributed equally to this work.
Mapping the functional versatility and fragility of Ras GTPase signaling circuits through in vitro network reconstitution.
eLife. 2016;5:12435 Jan. 14
Abstract. PDF.
Beyond editing: Repurposing CRISPR-Cas9 for precision genome regulation and interrogation
Nature Reviews Molecular Cell Biology. 2015 Dec 16
Abstract. PDF.
Oscillatory stress stimulation uncovers an Achilles' heel of the yeast MAPK signaling network
Science. 2015 Nov 19
Abstract. PDF.
Remote control of therapeutic T cells through a small molecule-gated chimeric receptor
Science. 2015 Sep 24
Abstract. PDF.
Synthetic biology approaches to engineer T cells
Current Opinion in Immunology. 2015; 35:123-130
Abstract. PDF.
Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds
Cell. 2015 Jan 15;160(1-2):339-50.
Abstract. PDF.
Specific Gene Repression by CRISPRi System Transferred through Bacterial Conjugation
ACS Synth Biol. 2014 Dec 19;3(12):929-31.
Abstract. PDF. Supplemental Data. Sending mixed messages for cell population control.
Cell (Preview) 158 : 973-975 (2014)
Abstract. PDF.
Synthetic control of mammalian-cell motility by engineering chemotaxis to an orthogonal bioinert chemical signal.
Proceedings of the National Academy of Sciences (April 2014)
Abstract. PDF. Supplemental Data. Supplemental Movie 1. Supplemental Movie 2. Supplemental Movie 3. Supplemental Movie 4. Supplemental Movie 5 Supplemental Movie 6.
Secreting and sensing the same molecule allows cells to achieve versatile social behaviors.
Science 343 (6171): 1242782 (2014)
Abstract. PDF. Supplemental Data.
Perspective in Science by Anna J. Lee and Linchong You (PDF)
Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module.
Cell 155(6):1422-34 (2013)
Abstract. PDF. Supplemental Data. Supplemental Movie 1. Supplemental Movie 2. RPPA data.
Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory.
Proceedings of the National Academy of Sciences 110(40):16015-16020 (2013)
Abstract. PDF. Supplemental Data.
Exploitation of Latent Allostery Enables the Evolution of New Modes of MAP Kinase Regulation.
Cell 154, 875-887 (2013)
Abstract. PDF. Supplemental Data.
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes.
Cell (Resource) 154, 1-10 (2013)
Abstract. PDF.
Cell-based therapeutics: the next pillar of medicine.
Science Translational Medicine 5(179) (2013)
Abstract. PDF.
Cell biology 2.0.
Trends Cell Biol. 22(12):611-2 (2013)
Abstract. PDF.
Design principles of regulatory networks: searching for the molecular algorithms of the cell.
Mol. Cell 49(2):202-12 (2013)
Abstract. PDF.
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression.
Cell 28;152(5):1173-83 (2013)
Abstract. PDF. Supplemental Data. Supplemental Sequences.
Designing synthetic regulatory networks capable of self-organizing cell polarization.
Cell 151(2):320-32 (2012)
Abstract. PDF. Supplemental Data. Supplemental Movie 1. Supplemental Movie 2. Simulation Code and Scripts.
Conformational Control of the Ste5 Scaffold Protein Insulates Against MAP Kinase Misactivation.
Science 337 (6099): 1218-1222 (2012)
Abstract. PDF. Supplemental Data.
Bacterial virulence proteins as tools to rewire kinase pathways in yeast and immune cells.
Nature 488, 384-388 (2012)
Abstract. PDF. Supplemental Data.
Systematic functional prioritization of protein posttranslational modifications.
Cell 150(2):413-25 (2012)
Abstract. PDF. Article + Supplemental Data. Supplemental Tables.
SYNZIP Protein Interaction Toolbox: in Vitro and in Vivo Specifications of Heterospecific Coiled-Coil Interaction Domains.
ACS Synth Biol. 1(4): 118-129 (2012)
Abstract. PDF. Supplemental Data.
Control of protein signaling using a computationally designed GTPase/GEF orthogonal pair.
Proceedings of the National Academy of Sciences 109(14): 5277-5282 (2012)
Abstract. PDF. Supplemental Data.
Light-based feedback for controlling intracellular signaling dynamics
Nature Methods 8, 837-839 (2011)
Abstract. PDF. Supplemental Data.
Recruitment interactions can override catalytic interactions in determining the functional identity of a protein kinase
Proceedings of the National Academy of Sciences 108, 9809-9814 (2011)
Abstract. PDF. Supplemental Data.
Light Control of Plasma Membrane Recruitment Using the Phy-PIF System
Methods Enzymology 497, 409-23 (2011)
Abstract. PDF.
Scaffold proteins: hubs for controlling the flow of cellular information
Science 332, 680-6 (2011)
Abstract. PDF. Supplemental Video.
The promise of optogenetics in cell biology: interrogating molecular circuits in space and time
Nature Methods 1, 35-38 (2011)
Abstract. PDF.
Build life to understand it
Nature 468, 889-890 (2010)
Abstract. PDF.
Phosphotyrosine signaling: evolving a new cellular communication system
Cell 142, 661-667 (2010)
Abstract. PDF.
The minimal autoinhibited unit of the guanine nucleotide exchange factor intersectin
PLos ONE 5, e11291 (2010)
Abstract. PDF.
Designing customized cell signalling circuits
Nature Reviews Molecular Cell Biology 11, 393-403 (2010)
Abstract. PDF.
Rapid diversification of cell signaling phenotypes by modular domain recombination
Science 328, 368-372 (2010)
Abstract. PDF. Supplemental Data.
Commentary in Nature Reviews Genetics by Katharine H. Wrighton (PDF) Nature Reviews Molecular Cell Biology by Katharine H. Wrighton (PDF)
Rewiring cells: synthetic biology as a tool to interrogate the organizational principles of living systems
Annual Review of Biophysics 39, 515-537 (2010)
Abstract. PDF. (Copyright Annual Reviews)
Reengineering the signaling properties of a Src family kinase
Biochemistry 48, 10956-10962 (2009)
Abstract. PDF. Supplemental Data.
Spatiotemporal control of cell signalling using a light-switchable protein interaction
Nature 461, 997-1001 (2009)
Abstract. PDF. Supplemental Data.
Defining network topologies that can achieve biochemical adaptation
Cell 138, 760-73 (2009)
Abstract. PDF. Supplemental Data. PaperFlick video summary.
Commentary in Cell by Alexander B. Artyukhin, Lani F. Wu, and Steven J. Altschuler (PDF)
Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species
PLoS Biology 7, e1000134 (2009).
Abstract. PDF. Supplemental Data.
The Ste5 Scaffold Directs Mating Signaling by Catalytically Unlocking the Fus3 MAP Kinase for Activation
Cell 136, 1085-97 (2009)
Abstract. PDF. Supplemental Data.
Commentary in Cell by Markus A. Seeliger and John Kuriyan (PDF)
The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency
Nature 454, 1005-8 (2008)
Abstract. PDF. Supplemental Data.
Evolution of the phospho-tyrosine signaling machinery in premetazoan lineages
Proceedings of the National Academy of Sciences 105, 9680-4 (2008)
Abstract. PDF. Supplemental Data.
Commentary in Proceedings of the National Academy of Sciences by Bruce Mayer (PDF)
Using engineered scaffold interactions to reshape MAP kinase pathway signaling dynamics
Science 319, 1539-43 (2008)
Abstract. PDF. Supplemental Data.
Commentary in Science by Peter M. Pryciak (PDF)Commentary in Nature Biotechnology by Patrick Guye and Ron Weiss (PDF)
Synthetic biology: lessons from the history of synthetic organic chemistry
Nature Chemical Biology 3, 521-5 (2007)
Abstract. PDF.
Rewiring cellular morphology pathways with synthetic guanine nucleotide exchange factors
Nature 447, 596-600 (2007)
Abstract. PDF. Supplemental Data. Supplemental Video.
Commentary in Molecular Systems Biology by Philippe Bastiaens (PDF)Commentary in Developmental Cell by Christopher S. Chen (PDF)
Engineering synthetic signaling proteins with ultrasensitive input/output control
Nature Biotechnology 25, 660-662 (2007)
Abstract. PDF. Supplemental Data.
Engineering modular protein interaction switches by sequence overlap
Journal of the American Chemical Society 129, 4606-4611 (2007)
Abstract. PDF. Supplemental Data.
Docking interactions in protein kinase and phosphatase networks
Current Opinion in Structural Biology 16, 676-685 (2006)
Abstract. PDF.
Domains, Motifs, and Scaffolds: The Role of Modular Interactions in the Evolution and Wiring of Cell Signaling Circuits
Annual Review of Biochemistry 75, 655-680 (2006)
Abstract. PDF.
(Copyright Annual Reviews)
The Ste5 Scaffold Allosterically Modulates Signaling Output of the Yeast Mating Pathway
Science 311, 822-826 (2006)
Abstract. PDF.
Commentary in Science by Ashton Breitkreutz and Mike Tyers (PDF)
The role of docking interactions in mediating discrimination in the yeast MAPK network
Molecular Cell 20, 951-962 (2005)
Abstract. PDF. Supplemental Data.
A general model for preferential hetero-oligomerization of Lin-2/7 domains: Mechanism underlying directed assembly of supramolecular signaling complexes
Journal of Biological Chemistry 280, 38528-38536 (2005)
Abstract. PDF.
A Polybasic Motif Allows N-WASP to Act as a Sensor of PIP2 Density
Molecular Cell 17, 181-191 (2005)
Abstract. PDF. Supplemental Data.
Commentary in Developmental Cell by Diana Murray and Barry Honig (PDF)
Rewiring cell signaling: the logic and plasticity of eukaryotic protein circuitry
Current Opinion in Structural Biology 14, 690-699 (2004)
Abstract. PDF.
Sho1 and Pbs2 Act as Coscaffolds Linking Components in the Yeast High Osmolarity MAP Kinase Pathway
Molecular Cell 14, 825–832 (2004)
Abstract. PDF. Supplemental Data.
Commentary in Current Biology by Bruce T. Seet and Tony Pawson (PDF)
Optimization of specificity in a cellular protein interaction network by negative selection
Nature 426, 676-680 (2003)
Abstract. PDF. Supplemental Data.
Commentary in Nature by Drew Endy and Michael B. Yaffe (PDF)
Reprogramming Control of an Allosteric Signaling Switch Through Modular Recombination
Science 301, 1904-1908 (2003)
Abstract. PDF. Supplemental Data.
The Structure and Function of Proline Recognition Domains
Science STKE 2003, re8 (2003)
Abstract. PDF. Structure Animations.
Role of Electrostatic Interactions in PDZ Domain Ligand Recognition
Biochemistry 42, 2797-2805 (2003)
Abstract. PDF.
Rewiring MAP Kinase Pathways Using Alternative Scaffold Assembly Mechanisms
Science 299, 1061-1064 (2003)v
Abstract. PDF. Supplemental Data.
Commentary in Science by Mark Ptashne and Alexander Gann (PDF) Commentary in Molecular Cell by James E. Ferrell, Jr. and Karlene A. Cimprich (PDF)
Structure of the N-WASP EVH1 Domain-WIP Complex: Insight into the Molecular Basis of Wiskott-Aldrich Syndrome
Cell 111, 565–576 (2002)
Abstract. PDF. Supplemental Data.
Coordinated folding and association of the LIN-2, -7 (L27) domain: An obligate heterodimerization involved in assembly of signaling and cell polarity complexes
Journal of Biological Chemistry 277, 34902-34908 (2002)
Abstract. PDF.
How signaling proteins integrate multiple inputs: a comparison of N-WASP and Cdk2
Current Opinion in Cell Biology14, 149-154 (2002)
Abstract. PDF.
The modular logic of signaling proteins: building allosteric switches from simple binding domains
Current Opinion in Structural Biology 12, 61-68 (2002)
Abstract. PDF.
Structure of the SH3-Guanylate Kinase Module from PSD-95 Suggests a Mechanism for Regulated Assembly of MAGUK Scaffolding Proteins
Molecular Cell 8, 1291-1301 (2001)
Abstract. PDF.
Commentary in Structure by Michael B. Yaffe (PDF)
Mechanism and role of PDZ domains in signaling complex assembly
Journal of Cell Science 114, 3219-3231 (2001)
Abstract. PDF.
The double life of PX domains
Nature Structural Biology 8, 570-572 (2001)
Abstract. PDF.
Energetic Determinants of Internal Motif Recognition by PDZ Domains
Biochemistry 40, 5921-5930 (2001)
Abstract. PDF.
Integration of Multiple Signals Through Cooperative Regulation of the N-WASP-Arp2/3 Complex
Science 290, 801-806 (2000)
Abstract. PDF. Supplemental Data.
Commentary in Science by James Fawcett and Tony Pawson (HTML)
Converging on proline: the mechanism of WW domain peptide recognition
Nature Structural Biology 7, 611-613 (2000)
Abstract. PDF.
Improving SH3 domain ligand selectivity using a non-natural scaffold
Chemistry & Biology 7, 463-473 (2000)
Abstract. PDF.
PSD-95 Assembles a Ternary Complex with the N-Methyl-D-aspartic Acid Receptor and a Bivalent Neuronal NO Synthase PDZ Domain
Journal of Biological Chemistry 274, 27467-27473 (1999)
Abstract. PDF.
Structure of the Enabled/VASP Homology 1 DomainPeptide Complex: A Key Component in the Spatial Control of Actin Assembly
Cell 97, 471-480 (1999)
Abstract. PDF.
Unexpected Modes of PDZ Domain Scaffolding Revealed by Structure of nNOS-Syntrophin Complex
Science 284, 812-815 (1999)
Abstract. PDF.
Commentary in Nature Structural Biology by Hartmut Oschkinat (PDF)
Exploiting the Basis of Proline Recognition by SH3 and WW Domains: Design of N-Substituted Inhibitors
Science 282, 2088-2092 (1998)
Abstract. PDF.
Commentary in Chemistry and Biology by Behzad Aghazadeh and Michael K. Rosen (PDF)