Spatiotemporal control of cell signalling using a light-switchable protein interaction
Supplementary Information
This file contains Supplementary Methods, Supplementary Table S1, Supplementary Data, Supplementary Movie Legends, Supplementary References and Supplementary Figures S1-S5 with Legends.
Movie S1
This movie shows YFP membrane recruitment - see Supplementary Information for full legend.
Movie S2
This movie shows YFP membrane dissociation - see Supplementary Information for full legend.
Movie S3
This movie shows oscillating YFP translocation - see Supplementary Information for full legend.
Movie S4
This movie shows YFP point recruitment - see Supplementary Information for full legend.
Movie S5
This movie shows patterned recruitment of YFP - see Supplementary Information for full legend.
Movie S6
This movie shows titrated recruitment of YFP - see Supplementary Information for full legend.
Movie S7
This movie shows cell extrusion by Tiam(Rac GEF) recruitment - see Supplementary Information for full legend.
Movie S8
This movie shows dynamic lamellipodia by Tiam(Rac GEF) - see Supplementary Information for full legend.
Movie S9
This movie shows periodic contractions by Tim(Rho GEF) recruitment - see Supplementary Information for full legend.
Movie S10
This movie shows PIF-YFP only recruitment control - see Supplementary Information for full legend.
Movie S11
This movie shows monitoring membrane-bound Cdc42-GTP dynamics via recruited mCherry-WASP-GBD in TIRF - see Supplementary Information for full legend.