Light-based feedback for controlling intracellular signaling dynamics
Nature Methods 8, 837-839 (2011)
Matlab toolbox for microscope control (via Micro-Manager) and the feedback controller |
Scaffold proteins: hubs for controlling the flow of cellular information
Science 332, 680-6 (2011)
Mechanisms of scaffold protein Ste5 (animation by Janet Iwasa ) |
File |
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Quicktime video |
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Windows Media video |
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MPEG-4 video |
Spatiotemporal control of cell signalling using a light-switchable protein interaction
Nature 461, 997-1001 (2009)
Supplementary Movies | File |
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Movie S1 - YFP memane recruitment |
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Movie S2 - YFP memane dissociation |
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Movie S3 - oscillating YFP translocation |
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Movie S4 - YFP point recruitment |
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Movie S5 - patterned recruitment of YFP |
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Movie S6 - titrated recruitment of YFP |
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Movie S7 - cell extrusion by Tiam (Rac GEF) recruitment |
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Movie S8 - dynamic lamellipodia by Tiam (Rac GEF) recruitment |
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Movie S9 - periodic contractions by Tim (Rho GEF) recruitment |
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Movie S10 - PIF-YFP-only recruitment control |
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Movie S11 - dynamics of memane-bound Cdc42-GTP in TIRF |
Cell 136, 1085-97 (2009)
Structure | File |
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'Minimal scaffold' (ms) domain of Ste5 |
The Ste5 Scaffold Allosterically Modulates Signaling Output of the YeastMating Pathway
Science 311, 822-826 (2006)
Structure | File |
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Fus3 in complex with a Ste5 peptide |
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Fus3 without a peptide from Ste5 |
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Fus3 phosphorylated on Tyr182 |
Molecular Cell 20, 951-962 (2005)
Structure | File |
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Mitogen activated protein kinase Fus3 (non-phosphorylated) |
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Fus3 with a docking motif from Ste7 |
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Fus3 with a docking motif from Msg5 |
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Fus3 with a docking motif from Far1 |
Cell 111, 565–576 (2002)
Structure | File |
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N-WASP EVH1 Domain - WIP complex |
Molecular Cell 8, 1291-1301 (2001)
Structure | File |
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SH3-guanylate kinase module from PSD-95 |
Cell 97, 471-480 (1999)
Structure | File |
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Enabled/VASP Homology 1 (EVH1) domain from mouse Enabled in complex with ActA peptide |
Unexpected Modes of PDZ Domain Scaffolding Revealed by Structure of nNOS-SyntrophinComplex
Science 284, 812-815 (1999)
Structure | File |
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Neuronal nitric oxide synthase (nNOS) PDZ domain |
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nNOS PDZ-syntrophin PDZ complex |
Exploiting the Basis of Proline Recognition by SH3 andWW Domains: Design of N-Substituted Inhibitors
Science 282, 2088-2092 (1998)
Structure | File |
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Sem5 C-term. SH3 in complex with Sos peptide |
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Sem5 C-term. SH3 in complex with isopropyl peptoid/peptide |
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Sem5 C-term. SH3 in complex with cyclopropyl methyl peptoid/peptide |
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Crk SH3 in complex with peptoid/peptide |